A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes
نویسندگان
چکیده
منابع مشابه
A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes
BACKGROUND To facilitate the screening of large quantities of new antimicrobial peptides (AMPs), we describe a cost-effective method for high throughput prokaryotic expression of AMPs. EDDIE, an autoproteolytic mutant of the N-terminal autoprotease, Npro, from classical swine fever virus, was selected as a fusion protein partner. The expression system was used for high-level expression of six a...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2012
ISSN: 1472-6750
DOI: 10.1186/1472-6750-12-10